Equipment

Renishaw Raman microscope

Research-grade Raman microscope with flexible capabilities
Excitation Wavelength: 785nm
Spatial Resolution: Diameter of sampled region depends on the diffraction limited performance of the microscope objective (sub-um)
Spectral Resolution: Better than 1cm-1
Spectral Range: Raman fingerprint (350-1800 cm-1) @ 785nm excitation

Credit: CHUM

Handheld line scanning Raman system

First large field of view Raman imaging system designed to be integrated to the workflow of surgical cancer resection and in vivo tissue margin assessment
Excitation Wavelength: 785nm
Hyperspectral detection of the fingerprint Raman signal
Sterilizable with field of view is 1 cm2
Spatial resolution ~250 µm
Allows visualization of tissue structures under white-light reflectance

[1] F. Daoust et al., “A handheld macroscopic Raman spectroscopy imaging instrument for machine learning based molecular tissue margins characterization,” under review for publication in J. Biomed. Opt. (2020).

Mesoscopic Raman probe

Hand held stainless steel probe that can be used for immersion or direct contact measurements
Excitation Wavelength: 785nm, 671nm
Spatial Resolution: Diameter of sampled region is ~500um
Spectral Resolution: Better than 10cm-1
Spectral Range: Raman fingerprint (350-1800 cm-1) @ 785nm excitation; Raman high wavenumber (2500-4000 cm-1) @ 671nm excitation

[1] J. Desroches et al., “Characterization of a Raman spectroscopy probe system for intraoperative brain tissue classification,” Biomed. Opt. Express 6, 2380 (2015).

[2] M. Jermyn et al., “Highly accurate detection of cancer in situ with intraoperative, label-free, multimodal optical spectroscopy,” Cancer Res. 77, 3942 (2017).